Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain EA31 and Saccharomyces cerevisiae strain SG | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D82Y | the mutant displays significantly decreased Km values and better kcat value compared to the wild type enzyme | Mus musculus |
L240M | the mutant's activity shows a drastic improvement compared with the wild type enzyme | Mus musculus |
Q62H | the mutant displays significantly decreased Km values and lower kcat value compared to the wild type enzyme | Mus musculus |
Q62H/S173N/L240M | the mutant's activity shows a drastic improvement compared with the wild type enzyme | Mus musculus |
S173N | the mutant displays significantly decreased Km values and better kcat value compared to the wild type enzyme | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.105 | - |
myo-inositol | mutant enzyme S173N, at 37°C, pH not specified in the publication | Mus musculus | |
2.516 | - |
myo-inositol | mutant enzyme D82Y/S173N/L240M, at 37°C, pH not specified in the publication | Mus musculus | |
2.905 | - |
myo-inositol | mutant enzyme Q62H, at 37°C, pH not specified in the publication | Mus musculus | |
3.573 | - |
myo-inositol | mutant enzyme D82Y, at 37°C, pH not specified in the publication | Mus musculus | |
4.55 | - |
myo-inositol | wild type enzyme, at 37°C, pH not specified in the publication | Mus musculus | |
7.629 | - |
myo-inositol | mutant enzyme L240M, at 37°C, pH not specified in the publication | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + O2 | Mus musculus | - |
D-glucuronate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9QXN5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + O2 | - |
Mus musculus | D-glucuronate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MIOX | - |
Mus musculus |
Myo-inositol oxygenase | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
59.5 | - |
myo-inositol | mutant enzyme Q62H, at 37°C, pH not specified in the publication | Mus musculus | |
76.8 | - |
myo-inositol | wild type enzyme, at 37°C, pH not specified in the publication | Mus musculus | |
85.1 | - |
myo-inositol | mutant enzyme S173N, at 37°C, pH not specified in the publication | Mus musculus | |
109.9 | - |
myo-inositol | mutant enzyme L240M, at 37°C, pH not specified in the publication | Mus musculus | |
110.8 | - |
myo-inositol | mutant enzyme D82Y/S173N/L240M, at 37°C, pH not specified in the publication | Mus musculus | |
129.4 | - |
myo-inositol | mutant enzyme D82Y, at 37°C, pH not specified in the publication | Mus musculus |